Add to anti-COMP wells

1. Standards 25 µl
   
   
2. Unknown samples 25 µl
   
   
3. Conjugate solution 100 µl
   
   
4. Incubate on a shaker for 2 hours at room temperature.
   
   
5. Wash 6 times with automatic washer or Aspirate the reaction volume. Add 350 µl Washing solution to each well. Aspirate completely. Repeat 5 times. After final wash, invert and tap the plate firmly against absorbent paper.
   
   
6. Add 200 µl Peroxidase substrate.
   
   
7. Incubate for 15 minutes.
   
   
8. Add 50 µl Stop solution. Put the plate on the shaker for approximately 5 seconds to ensure mixing of substrate and stop solution.
   
   
9. Measure the absorbance at 450 nm and evaluate.